ESI BIO的使命是成為客戶信賴的合作伙伴，將科學(xué)發(fā)現(xiàn)轉(zhuǎn)化為診所。我們通過(guò)提供突破性的干細(xì)胞技術(shù)作為研究級(jí)和治療級(jí)產(chǎn)品來(lái)實(shí)現(xiàn)這一目標(biāo)，從研究到診所的照明，以幫助我們的客戶實(shí)現(xiàn)他們治療疾病的目標(biāo)。
ESI BIO建立在豐富的創(chuàng)新和科學(xué)的基礎(chǔ)之上。我們于2000年在新加坡成立了ES Cell International，在那里我們通過(guò)制造個(gè)可翻譯的臨床級(jí)胚胎干細(xì)胞系來(lái)創(chuàng)建歷史，以便分發(fā)給研究界。
ESI BIO現(xiàn)在開(kāi)展業(yè)務(wù)Alameda CA. 我們是BioTime，Inc。擁有的以干細(xì)胞為基礎(chǔ)的子公司的驕傲家族的成員，我們從中受益于的科學(xué)，知識(shí)產(chǎn)權(quán)和干細(xì)胞技術(shù)的深層來(lái)源。
Product Name | SKU | Price |
HyStem®-C Hydrogel Trial Kit, 2.5 mL | GS312 | $85.00 |
HyStem®-C Hydrogel Trial Kit w/ PEGSSDA, 2.5 mL | GS312P | $125.00 |
HyStem®-C Hydrogel Kit, 7.5 mL | GS313 | $195.00 |
HyStem®-C Hydrogel Kit w/ PEGSSDA, 7.5 mL | GS313P | $225.00 |
HyStem®-C Hydrogel Kit, 12.5 mL | GS1005 | $245.00 |
HyStem ® -C水凝膠為用于細(xì)胞培養(yǎng)基質(zhì)優(yōu)化提供一個(gè)很好的起點(diǎn)。HyStem-C*化學(xué)成分確定的和基于三個(gè)生物相容性組件：巰基修飾的透明質(zhì)酸（Glycosil），巰基反應(yīng)性交聯(lián)劑，PEGDA（Extralink），以及巰基修飾的膠原變性（格林-S ®）。Gelin-S為各種原代細(xì)胞和細(xì)胞系提供了基本的細(xì)胞附著位點(diǎn)，因此被推薦作為貼壁細(xì)胞類型和細(xì)胞培養(yǎng)優(yōu)化的理想底物。在某些情況下，可以通過(guò)添加ECM蛋白來(lái)進(jìn)一步增強(qiáng)HyStem-C水凝膠以匹配天然信號(hào)。
特征
· 水凝膠適用于培養(yǎng)原代細(xì)胞，干細(xì)胞和細(xì)胞系。
· 細(xì)胞可以以任何形式包封或生長(zhǎng)在水凝膠表面上，包括培養(yǎng)瓶，6-至384孔板或組織培養(yǎng)插入物。
· 通過(guò)操縱組分濃度和混合比，使用者可以容易地定制水凝膠以具有所需的硬度和凝膠化時(shí)間。
· 可定制的凝膠特性，包括凝膠時(shí)間和水凝膠硬度。
凝膠化
重組的HyStem-C組分在15至37℃保持液態(tài)。當(dāng)交聯(lián)劑，Extralink在水凝膠形成®（PEGDA）加入到Glycosil的混合物®（巰基修飾的透明質(zhì)酸）和格林-S ®（巰基修飾的明膠）。在所有三種組分混合后約20分鐘發(fā)生凝膠化。沒(méi)有步驟取決于低溫或低pH。用磷酸鹽緩沖鹽水（PBS）或細(xì)胞培養(yǎng)基稀釋組分可以增加凝膠化時(shí)間。
3D細(xì)胞恢復(fù)矩陣
對(duì)于細(xì)胞回收至關(guān)重要的應(yīng)用，替代交聯(lián)劑PEGSSDA可用于所有HyStem，HyStem-C和HyStem-HP試劑盒。這種交聯(lián)劑具有Extralink提供的相同優(yōu)點(diǎn)，還具有易于還原的內(nèi)部鍵的額外優(yōu)點(diǎn)。這允許從水凝膠中快速，容易地回收單細(xì)胞或簇，用于RNA分析或流式細(xì)胞術(shù)等應(yīng)用，而不是可影響細(xì)胞活力的慢酶法。鼓勵(lì)研究人員與我們聯(lián)系，以確定特定細(xì)胞類型或培養(yǎng)系統(tǒng)與PEGSSDA的相容性。
儲(chǔ)存和穩(wěn)定性 | 將Glycosil和Gelin-S在-20°C的原始樣品瓶中保存長(zhǎng)達(dá)一年。 |
技術(shù)文件 | GS312技術(shù)數(shù)據(jù)表 產(chǎn)品支持 HyStem Hydrogels常見(jiàn)問(wèn)題解答 小冊(cè)子 HyStem ®水凝膠 |
組件 | Extralink ® |
參考 | 新！Gaetani，R.，et al。（2015）心臟祖細(xì)胞在3D打印的明膠/透明質(zhì)酸貼劑中的心外膜應(yīng)用保留了心肌梗塞后的心臟功能。Biomaterials 61：339-348。PMID：17335875。Prestwich |
ESI BIO產(chǎn)品目錄：
貨號(hào) | 品名 | 規(guī)格 | 品牌 |
GS310 | HyStem® Hydrogel Kit | 2.5 mL | ESIBIO |
GS311 | HyStem® Hydrogel Kit | 7.5 mL | ESIBIO |
GS1004 | HyStem® Hydrogel Kit | 12.5 mL | ESIBIO |
GS310P | HyStem® Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS311P | HyStem® Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS312 | HyStem®-C Hydrogel Kit | 2.5 mL | ESIBIO |
GS313 | HyStem®-C Hydrogel Kit | 7.5 mL | ESIBIO |
GS1005 | HyStem®-C Hydrogel Kit | 12.5 mL | ESIBIO |
GS312P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS313P | HyStem®-C Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS314 | HyStem®-HP Hydrogel Kit | 2.5 mL | ESIBIO |
GS315 | HyStem®-HP Hydrogel Kit | 7.5 mL | ESIBIO |
GS1006 | HyStem®-HP Hydrogel Kit | 12.5 mL | ESIBIO |
GS314P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 2.5 mL | ESIBIO |
GS315P | HyStem®-HP Hydrogel Kit w/ PEGSSDA | 7.5 mL | ESIBIO |
GS1007 | HyStem® Hydrogel UV QuickSet Kit | 2.5 mL | ESIBIO |
GS1008 | HyStem® Hydrogel UV QuickSet Kit | 7.5 mL | ESIBIO |
GS450 | PEGgel Kit | 1 mL | ESIBIO |
GS240 | DG Water | 10 mL | ESIBIO |
GS241 | DG Water | 20 mL | ESIBIO |
GS3007 | Extralink® Vial | 0.5 mL | ESIBIO |
GS3006 | Extralink® Vial | 2.5 mL | ESIBIO |
GS3009 | Extralink® Lite Vial | 0.5 mL | ESIBIO |
GS3008 | Extralink® Lite Vial | 2.5 mL | ESIBIO |
5050 | Fibronectin | 1 mg | ESIBIO |
GS231 | Gelin-S® Thiol-modified Gelatin | 1 mL | ESIBIO |
GS230 | Gelin-S® Thiol-modified Gelatin | 5 mL | ESIBIO |
GS222 | Glycosil® Hyaluronic Acid | 1 mL | ESIBIO |
GS220 | Glycosil® Hyaluronic Acid | 5 mL | ESIBIO |
GS217 | Heprasil® Hyaluronic Acid | 1 mL | ESIBIO |
GS215 | Heprasil® Hyaluronic Acid | 5 mL | ESIBIO |
5010-D | Nutragen® Bovine Collagen | 50 mL | ESIBIO |
GS711 | PEGDA | 1 mL | ESIBIO |
GS700 | PEGDA | 1 g | ESIBIO |
GS705 | PEGDA | 5 g | ESIBIO |
GS755 | PEGSSDA | 0.5 mL | ESIBIO |
5020 | PEPTITE-2000® RGD Peptide | 5 mg | ESIBIO |
5005-B | PureCol® Collagen | 100 mL | ESIBIO |
5007-A | VitroCol® Collagen | 20 mL | ESIBIO |
5051 | Vitronectin | 0.1 mg | ESIBIO |
EM2203 | ExoSense™ CD63 Exosome ELISA Kit | 96 assays | ESIBIO |
EM2202 | VascuNet™ CoCulture Assay Kit | 1 Kit | ESIBIO |
ST11006 | BioLiteTM SSEA-1 (DyLight 488) anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11008 | BioLiteTM TRA-1-81 (DyLight 488) anti-Human Antibody | 100 µL | ESIBIO |
ST11023 | Nestin anti-Human Antibody | 100 µL | ESIBIO |
ST11003 | Oct4 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11001 | Sox2 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11013 | SSEA-1 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11014 | SSEA-3 anti-Human/Mouse Antibody | 100 µL | ESIBIO |
ST11015 | SSEA-4 anti-Human Antibody | 100 µL | ESIBIO |
ST11018 | TRA-1-60 (PE) anti-Human Antibody | 100 µL | ESIBIO |
ST11016 | TRA-1-60 anti-Human Antibody | 100 µL | ESIBIO |
ST11017 | TRA-1-81 anti-Human Antibody | 100 µL | ESIBIO |
ST12007 | LIF, Mouse Recombinant | 10 µg | ESIBIO |
ST12008 | LIF, Mouse Recombinant | 100 µg | ESIBIO |
ES-84 | PureStem® 4D20.8, NCr-fac Progenitor | ea | ESIBIO |
ES-98 | PureStem® E15, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-283 | PureStem® 7PEND24, NCr-fac Progenitor | ea | ESIBIO |
ES-278 | PureStem® 7SMOO32, NCr-fac Progenitor | ea | ESIBIO |
ES-250 | PureStem® SK11, NCr-fac Progenitor | ea | ESIBIO |
ES-268 | PureStem® MEL2, NCr-fac Progenitor | ea | ESIBIO |
ES-256 | PureStem® SM30, NCr-fac Progenitor | ea | ESIBIO |
ES-335 | PureStem® ES-335 Meso-latp Progenitor | ea | ESIBIO |
ES-100 | PureStem® E72 BETATROPHIN+ Progenitor | ea | ESIBIO |
ES-1001 | PureStem® ES-1001 GDF11+ Progenitor | ea | ESIBIO |
ES-184 | PureStem® EN7 Progenitor | ea | ESIBIO |
ES-101 | PureStem® ES-101 Progenitor | ea | ESIBIO |
ES-139 | PureStem® ES-139 Progenitor | ea | ESIBIO |
ES-154 | PureStem® ES-154 Progenitor | ea | ESIBIO |
ES-198 | PureStem® ES-198 Progenitor | ea | ESIBIO |
ES-199 | PureStem® ES-199 Progenitor | ea | ESIBIO |
ES-209 | PureStem® ES-209, Meso-prx/latp Progenitor | ea | ESIBIO |
ES-210 | PureStem® ES-210, Ecto-ntu Progenitor | ea | ESIBIO |
ES-236 | PureStem® ES-236 Progenitor | ea | ESIBIO |
ES-170 | PureStem® E44 Progenitor | ea | ESIBIO |
ES-196 | PureStem® W10 Progenitor | ea | ESIBIO |
ES-194 | PureStem® Z11, Meso Progenitor | ea | ESIBIO |
EM-1001 | PureStem® Progenitor Growth Media, EPM k01 | 500 mL | ESIBIO |
EM-1002 | PureStem® Progenitor Growth Media, EPM k02 | 500 mL | ESIBIO |
EM-1003 | PureStem® Progenitor Growth Media, EPM k03 | 500 mL | ESIBIO |
EM-1004 | PureStem® Progenitor Growth Media, EPM k04 | 500 mL | ESIBIO |
EM-1005 | PureStem® Progenitor Growth Media, EPM k05 | 500 mL | ESIBIO |
EM-1006 | PureStem® Progenitor Growth Media, EPM k06 | 500 mL | ESIBIO |
EM-1007 | PureStem® Progenitor Growth Media, EPM k07 | 500 mL | ESIBIO |
EM-1008 | PureStem® Progenitor Growth Media, EPM k08 | 500 mL | ESIBIO |
EM-2002 | HyStem®-4D Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2007 | HyStem®-4D Differentiation Kit | 1 Kit | ESIBIO |
EM-2001 | PureStem® Chondrogenesis Differentiation Kit | 1 Kit | ESIBIO |
EM-2006 | PureStem® Choroid Plexus Differentiation Kit | 1 Kit | ESIBIO |
EM-2003 | PureStem® Osteogenesis Differentiation Kit 01 | 1 Kit | ESIBIO |
EM-2004 | PureStem® Osteogenesis Differentiation Kit 02 | 1 Kit | ESIBIO |
ST10035 | LY411575 | 5 mg | ESIBIO |
ST10008 | PD0325901 | 2 mg | ESIBIO |
ST10009 | PD0325901 | 10 mg | ESIBIO |
ST10010 | PD0325901 | 415 µL, 10 mM | ESIBIO |
ST10034 | PD173074 | 2 mg | ESIBIO |
ST10021 | RepSox | 5 mg | ESIBIO |
ST10027 | RG108 | 5 mg | ESIBIO |
ST10024 | SB203580 | 2 mg | ESIBIO |
ST10012 | SB431542 | 5 mg | ESIBIO |
ST10013 | SB431542 | 10 mg | ESIBIO |
ST10014 | SB431542 | 1.3 mL, 10 mM | ESIBIO |
ST10025 | SP600125 | 5 mg | ESIBIO |
ST10015 | Thiazovivin | 2 mg | ESIBIO |
ST10017 | XAV939 | 2 mg | ESIBIO |
ST10018 | Y27632 | 2 mg | ESIBIO |
ST10019 | Y27632 | 10 mg | ESIBIO |
ST10020 | Y27632 | 625 µL, 10 mM | ESIBIO |
上海起發(fā)實(shí)驗(yàn)試劑有限公司是實(shí)驗(yàn)試劑一站式采購(gòu)服務(wù)商
1：強(qiáng)大的進(jìn)口輻射能力，血清、抗體、耗材、大部分限制進(jìn)口品等。
2：產(chǎn)品種類齊全，經(jīng)營(yíng)超過(guò)700多品牌，基本涵蓋所有生物實(shí)驗(yàn)試劑耗材。
3：提供加急服務(wù)，貨品一般1-2周到貨。
4：富有競(jìng)爭(zhēng)力的價(jià)格優(yōu)勢(shì)，絕大部分價(jià)格有優(yōu)勢(shì)。
5：多年積累良好的信譽(yù)，大部分客戶提供貨到付款服務(wù)?？蛻舭ㄇ迦A、北大、交大、復(fù)旦、中山等100多所高校，ROCHE，阿斯利康、國(guó)藥、fisher等知藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國(guó)外公司授權(quán)代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發(fā)，歡迎合作。
